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Proceedings Paper

Raman and fluorescence spectroscopic evaluation of NIR laser welded human and porcine aorta tissues
Author(s): Alvin Katz; Alexandra Alimova; Glenn Minko; C. H. Liu; Tapan K. Gayen; Howard E. Savage; Rabindra K. Halder; Steven A. McCormick; Robert R. Alfano
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Paper Abstract

Laser tissue welding involves the partial denaturing and renaturing of the collagen triple helical structure. Although the mechanisms of laser tissue welding are not well understood, water in tissues plays an important role in the process. High quality welding of human and porcine aorta tissue have been achieved using NIR lasers tuned to the water absorption band around 1450 nm. Fluorescence and Raman spectra from welded and non-welded regions are compared for ex vivo human and porcine aorta tissues. The fluorescence from the outer surface of welded aorta was substantially weaker than the fluorescence from the non-welded region. The Raman spectra from the welded and non-welded tissue regions appeared similar in the energies of the observed vibrational levels but the intensity of the fluorescence wing was considerably greater from the outer surface of the welded region as compared to the non-welded region. For the outer surface of the aorta, the emission intensity from the welded region was larger than for the non-welded region.

Paper Details

Date Published: 12 September 2003
PDF: 4 pages
Proc. SPIE 4949, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems XIII, (12 September 2003); doi: 10.1117/12.476390
Show Author Affiliations
Alvin Katz, Institute for Ultrafast Spectroscopy and Lasers, City College of New York (United States)
Alexandra Alimova, Institute for Ultrafast Spectroscopy and Lasers, City College of New York (United States)
Glenn Minko, Institute for Ultrafast Spectroscopy and Lasers, City College of New York (United States)
C. H. Liu, Institute for Ultrafast Spectroscopy and Lasers, City College of New York (United States)
Tapan K. Gayen, Institute for Ultrafast Spectroscopy and Lasers, City College of New York (United States)
Howard E. Savage, New York Eye and Ear Infirmary (United States)
Rabindra K. Halder, Institute for Ultrafast Spectroscopy and Lasers, City College of New York (United States)
Steven A. McCormick, New York Eye and Ear Infirmary (United States)
Robert R. Alfano, Institute for Ultrafast Spectroscopy and Lasers, City College of New York (United States)


Published in SPIE Proceedings Vol. 4949:
Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems XIII
Eugene A. Trowers; Timothy A. Woodward; Werner T.W. de Riese; Lawrence S. Bass; Nikiforos Kollias; Udayan K. Shah; Brian Jet-Fei Wong; Reza S. Malek; David S. Robinson; Hans-Dieter Reidenbach; Keith D. Paulsen; Kenton W. Gregory; Lawrence S. Bass; Abraham Katzir, Editor(s)

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