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Proceedings Paper

Performance evaluation of fiber optic probes for tissue lifetime fluorescence spectroscopy
Author(s): Thanassis Papaioannou; Norris W. Preyer Jr.; Qiyin Fang; Hamza Kurt; Michael Carnohan; Russel Ross; Adam Brightwell; Greg Cottone; Linda Ramball Jones; Laura Marcu
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Paper Abstract

The design of fiber-optic probes plays an important role in optical spectroscopic studies, including fluorescence spectroscopy of biological tissues. It can affect the light delivery and propagation into the tissue, the collection efficiency (total number of photons collected vs. total number of photons launched) and the origin of collected light. This in turn affects the signal to noise ratio (SNR) and the extend of tissue interrogation, thus influencing the diagnostic value of such techniques. Three specific fiber-optic probe designs were tested both experimentally and computationally via Monte Carlo simulations. In particular, the effects of probe architecture (single-fiber vs. two bifurcated multifiber probes), probe-to-target distance (PTD), and source-to-detector separation (SDS) were investigated on the collected diffuse reflectance of a Lambertian target and an agar-based tissue phantom. This study demonstrated that probe architecture, PTD, and SDS are closely intertwined and considerably affect the light collection efficiency, the extend of target illumination, and the origin of the collected reflected light. Our findings can be applied towards optimization of fiber-optic probe designs for quantitative fluorescence spectroscopy of diseased tissues.

Paper Details

Date Published: 22 July 2003
PDF: 8 pages
Proc. SPIE 4958, Advanced Biomedical and Clinical Diagnostic Systems, (22 July 2003); doi: 10.1117/12.476141
Show Author Affiliations
Thanassis Papaioannou, Cedars-Sinai Medical Ctr. (United States)
Norris W. Preyer Jr., College of Charleston (United States)
Qiyin Fang, Cedars-Sinai Medical Ctr. (United States)
Hamza Kurt, Cedars-Sinai Medical Ctr. (United States)
College of Charleston (United States)
Michael Carnohan, College of Charleston (United States)
Russel Ross, College of Charleston (United States)
Adam Brightwell, College of Charleston (United States)
Greg Cottone, College of Charleston (United States)
Linda Ramball Jones, College of Charleston (United States)
Laura Marcu, Cedars-Sinai Medical Ctr. (United States)
Univ. of Southern California (United States)

Published in SPIE Proceedings Vol. 4958:
Advanced Biomedical and Clinical Diagnostic Systems
Tuan Vo-Dinh; Warren S. Grundfest M.D.; David A. Benaron M.D.; Gerald E. Cohn, Editor(s)

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