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Proceedings Paper

Compact time-resolved laser-induced fluorescence spectroscopic system for clinical investigations of diseased tissues
Author(s): Qiyin Fang; Thanassis Papaioannou; Javier Jo; Russel Vaitha; Kumar Shastry; Laura Marcu
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Paper Abstract

In this communication, we report the design, development, calibration, and characterization of a fluorescence spectroscopy instrument capable of measuring time-resolved fluorescence spectra from 350nm to 800nm with subnanosecond resolution. The compact system can be used in a variety of clinical settings including endoscopic procedures. The instrument can accommodate various types of laser sources and fiber optic probes for different types of applications. The typical acquisition time is about 0.8 s per wavelength or 40 s for a 200 nm time-resolved fluorescence spectrum including online fluorescence lifetime analysis. The system has a variable spectral resolution from 0.1 to 10 nm and temporal resolution of 300 ps. Fluorescence spectra can be acquired with a sensitivity of 10-9M at signal-to-noise ratio of 46. The fluorescence lifetimes of Rhodamin B and 9- cyanoanthracene were determined to be 2.92∓0.12 ns and 12.28∓0.12 ns indicating good agreement with literature values.

Paper Details

Date Published: 22 July 2003
PDF: 7 pages
Proc. SPIE 4958, Advanced Biomedical and Clinical Diagnostic Systems, (22 July 2003); doi: 10.1117/12.476133
Show Author Affiliations
Qiyin Fang, Cedars-Sinai Medical Ctr. (United States)
Thanassis Papaioannou, Cedars-Sinai Medical Ctr. (United States)
Javier Jo, Cedars-Sinai Medical Ctr. (United States)
Russel Vaitha, Cedars-Sinai Medical Ctr. (United States)
Kumar Shastry, Cedars-Sinai Medical Ctr. (United States)
Laura Marcu, Cedars-Sinai Medical Ctr. (United States)
University of Southern California (United States)

Published in SPIE Proceedings Vol. 4958:
Advanced Biomedical and Clinical Diagnostic Systems
Tuan Vo-Dinh; Warren S. Grundfest; David A. Benaron; Gerald E. Cohn, Editor(s)

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