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Proceedings Paper

Refractive index of escherichia coli cells
Author(s): Aleksei E. Balaev; K. N. Dvoretski; Valeri A. Doubrovski
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Paper Abstract

Experimental values of the extinction of Escherichia coli cells water suspension by Bryant et al. are used for the refractive index determination of these bacteria. The real suspension of E. coli cells is modeled as a system of identical randomly oriented homogeneous circular cylinders. This model permits one to determine the volume average value of the refractive index of the optically soft particles with a narrow size distribution. The discrete Shifrin method is used to calculate the extinction cross section of the finite cylinders. The results indicate that the relative refractive index is practically independent of the wavelength of the incident radiation in the range from 0.4 micrometers to 0.8 micrometers . The mean value of 1.0387 in this range corresponds to the absolute refractive index value equal to 1.384 at 0.589 micrometers . The obtained value of refractive index of E. coli cells is somewhat lower than the result of immersion refractometry measured by Bryant et al.

Paper Details

Date Published: 16 July 2002
PDF: 8 pages
Proc. SPIE 4707, Saratov Fall Meeting 2001: Optical Technologies in Biophysics and Medicine III, (16 July 2002); doi: 10.1117/12.475627
Show Author Affiliations
Aleksei E. Balaev, Saratov State Medical Univ. (Russia)
K. N. Dvoretski, Saratov State Medical Univ. (Russia)
Valeri A. Doubrovski, Saratov State Medical Univ. (Russia)

Published in SPIE Proceedings Vol. 4707:
Saratov Fall Meeting 2001: Optical Technologies in Biophysics and Medicine III
Valery V. Tuchin, Editor(s)

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