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Proceedings Paper

Liposomes by quasielastic light scattering and spectroturbidimetry
Author(s): Boris N. Khlebtsov; Nikolai G. Khlebtsov; Sergei Y. Shchyogolev
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Paper Abstract

A variant of the experimental implementation of the quasi- elastic light scattering (QELS) method for determining the average particle size in liposome suspensions in the homodyne mode was considered. The aim of the investigations was to compare the data obtained with the result of a version of the method of spectroturbidimetry (STM) previously developed to determine the size and shell thickness of liposomes. For determination of the corresponding correlation functional, an experimental setup was used that was base don a helium-neon laser with a computer sound card as the analog-digital converter. Monodisperse suspension of latexes and colloidal gold as well as E. coli cell suspension were used as the test objects. The tests showed good accuracy of the QELS determination of the particle diameter d in the region of d < 100. Below this boundary, the accuracy of the particle size determination is limited by the relatively low resolving capacity of the analog-digital converter of the given type. It was established that the results of the determination of the average particle size in liposome polydisperse suspension obtained by QELS and STM were in satisfactory agreement.

Paper Details

Date Published: 16 July 2002
PDF: 5 pages
Proc. SPIE 4707, Saratov Fall Meeting 2001: Optical Technologies in Biophysics and Medicine III, (16 July 2002); doi: 10.1117/12.475603
Show Author Affiliations
Boris N. Khlebtsov, Institute of Biochemistry and Physiology of Plants and Microorganisms (Russia)
Nikolai G. Khlebtsov, Institute of Biochemistry and Physiology of Plants and Microorganisms and Saratov State Un (Russia)
Sergei Y. Shchyogolev, Institute of Biochemistry and Physiology of Plants and Microorganisms and Saratov State Un (Russia)


Published in SPIE Proceedings Vol. 4707:
Saratov Fall Meeting 2001: Optical Technologies in Biophysics and Medicine III

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