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Proceedings Paper

Study of the intensity ratio from the characteristics of autofluorescence spectra of the human colorectal tissue
Author(s): Tianyun Zhao; Teck-Chee Chia; Cheong Hoong Diong
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Paper Abstract

Autofluorescence is widely investigated as a sensitive method in early diagnosis of diseased tissue, but most measurements of tissue autofluorescence were performed with only a single excitation wavelength. This work is aim to optimize the excitation wavelength or emission wavelength bands for fluorescence spectroscopy for better clinical diagnostic accuracy. The autofluorescence spectra of colorectal tissue were studied over a wide excitation range (350-600 nm). The excitation 350 nm and 470 nm were identified as effective excitation wavelength for inducing tissue autofluorescence. The ratio of emission peak intensity at 470 nm versus 610 nm excited by 350 nm and the ratio of emission peak intensity at 510 nm versus 610 nm excited by 470 nm were found as good indications to distinguish normal and diseased tissue. The observed peaks of the fluorescence spectra were also compared with some well known tissue fluorophores.

Paper Details

Date Published: 17 February 2003
PDF: 10 pages
Proc. SPIE 4833, Applications of Photonic Technology 5, (17 February 2003); doi: 10.1117/12.474747
Show Author Affiliations
Tianyun Zhao, Nanyang Technological University (Singapore)
Teck-Chee Chia, Nanyang Technological Univ. (Singapore)
Cheong Hoong Diong, Nanyang Technological Univ. (Singapore)

Published in SPIE Proceedings Vol. 4833:
Applications of Photonic Technology 5
Roger A. Lessard; George A. Lampropoulos; Gregory W. Schinn, Editor(s)

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