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Stimulation of chondrocyte proliferation following photothermal, thermal, and mechanical injury in ex-vivo cartilage grafts
Author(s): Nidhi S. Pandoh; Mai Thy Truong; Sergio H. Diaz-Valdes; David M. Gardiner; Brian Jet-Fei Wong
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Paper Abstract

Laser irradiation may stimulate chondrocytes proliferation in the peripheral region surrounding a photothermally-heated area in rabbit nasal septal cartilage. In this study, ex- vivo rabbit nasal septal cartilages maintained in culture were irradiated with an Nd:YAG laser ((lambda) equals1.32 micrometers , 4-16 sec, 10-45 W/cm2) to examine the relationship between the diameter of replicating cells and irradiation time. Also, this study investigated whether proliferation occurs following heating (by immersion in hot saline baths, with a heated metal rod, and a soldering iron) and mechanical modification (crushing with a metal stamp and scoring with a scalpel). Replicating chondrocytes were identified using a Bromodeoxyuridine (BrdU) double antibody detection system in whole mount tissue. Light microscopy was used to confirm the presence of BrdU stained chondrocytes. The mechanical and thermal stressors used failed to produce a proliferative response in chondrocytes as previously seen with laser irradiation. We suspect that chondrocyte proliferation may be induced as a response to alteration in matrix structure produced by photothermal, thermal, or mechanical modification of the matrix. Heat generated by a laser to stimulate chondrocyte proliferation may lead to new treatment options for degenerative articular diseases and disorders. Laser technology can be adapted for use with minimally invasive surgical instrumentation to deliver light into otherwise inaccessible regions of the body.

Paper Details

Date Published: 27 June 2002
PDF: 8 pages
Proc. SPIE 4617, Laser Tissue Interaction XIII: Photochemical, Photothermal, and Photomechanical, (27 June 2002); doi: 10.1117/12.472537
Show Author Affiliations
Nidhi S. Pandoh, Beckman Laser Institute and Medical Clinic (United States)
Mai Thy Truong, Beckman Laser Institute and Medical Clinic (United States)
Sergio H. Diaz-Valdes, Beckman Laser Institute and Medical Clinic (United States)
David M. Gardiner, Univ. of California/Irvine (United States)
Brian Jet-Fei Wong, Beckman Laser Institute and Medical Clinic (United States)


Published in SPIE Proceedings Vol. 4617:
Laser Tissue Interaction XIII: Photochemical, Photothermal, and Photomechanical
Steven L. Jacques; Donald Dean Duncan; Sean J. Kirkpatrick; Andres Kriete; Donald Dean Duncan; Sean J. Kirkpatrick; Andres Kriete, Editor(s)

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