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Proceedings Paper

Hydrogen peroxide production in cultured RPE cells exposed to near-infrared lasers
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Paper Abstract

Hydrogen peroxide (H2O2) in situ synthesis within cultures of retinal pigment epithelial cells exposed to near-IR (NIR) continuous wave (CW) and mode-locked lasers is investigated. Any production of H2O2 as a consequence of NIR laser exposure is indicative of a multiphoton absorption event, resulting in an intracellular photochemical reaction. Comparisons of mode-locked and CW laser threshold energies required for the photo-oxidative reaction provide support for an enhancement in multiphoton absorption in tissues exposed to the high peak powers inherent in mode-locked laser beams. Due to different fluorescent staining properties our results indicate different mechanisms for oxidative stress from CW and mode- locked laser exposure.

Paper Details

Date Published: 27 June 2002
PDF: 6 pages
Proc. SPIE 4617, Laser Tissue Interaction XIII: Photochemical, Photothermal, and Photomechanical, (27 June 2002); doi: 10.1117/12.472518
Show Author Affiliations
Michael L. Denton, Northorp Grumman Corp. (United States)
Debbie M. Eikum, Northorp Grumman Corp. (United States)
David J. Stolarski, Northorp Grumman Corp. (United States)
Gary D. Noojin, Northorp Grumman Corp. (United States)
Robert J. Thomas, Northorp Grumman Corp. (United States)
Randolph D. Glickman, Univ. of Texas Health Science Ctr./San Antonio (United States)
Benjamin A. Rockwell, Air Force Research Lab. (United States)

Published in SPIE Proceedings Vol. 4617:
Laser Tissue Interaction XIII: Photochemical, Photothermal, and Photomechanical
Steven L. Jacques; Donald Dean Duncan; Donald Dean Duncan; Sean J. Kirkpatrick; Sean J. Kirkpatrick; Andres Kriete; Andres Kriete, Editor(s)

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