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Proceedings Paper

Use of steady-state fluorescence anisotropy with pebble nanosensors for chemical analysis
Author(s): Thomas Horvath; Eric E. Monson; James Sumner; Hao Xu; Raoul Kopelman
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Paper Abstract

In this study we show that steady-state fluorescence anisotorpy within PEBBLEs can be used for the optochemical sensing of analytes such as Zn2+, O2, and Ca2+. Steady-state fluorescence anisotropy is a non- time resolved method that measures a combination of rotational and fluorescence lifetimes. This eliminates the need for reference dyes and ratiometic techniques to obtain quantitative results, even when using intensity-based sensor dyes. An advantage to working with PEBBLE nanosensors is that the encapsulated dye is localized in a constant rotational environment. This is in contrast to the use of free dyes, which can be affected by interferents such as protein binding.

Paper Details

Date Published: 21 June 2002
PDF: 7 pages
Proc. SPIE 4626, Biomedical Nanotechnology Architectures and Applications, (21 June 2002); doi: 10.1117/12.472115
Show Author Affiliations
Thomas Horvath, Univ. of Michigan (United States)
Eric E. Monson, Univ. of Michigan (United States)
James Sumner, Univ. of Michigan (United States)
Hao Xu, Univ. of Michigan (United States)
Raoul Kopelman, Univ. of Michigan (United States)

Published in SPIE Proceedings Vol. 4626:
Biomedical Nanotechnology Architectures and Applications
Raymond P. Mariella; Michelle Palmer; Darryl J. Bornhop; Darryl J. Bornhop; Ramesh Raghavachari; Shuming Nie; Ramesh Raghavachari; Catherine J. Murphy; David A. Dunn; David A. Dunn; Raymond P. Mariella; Catherine J. Murphy; Dan V. Nicolau; Shuming Nie; Michelle Palmer; Ramesh Raghavachari, Editor(s)

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