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Proceedings Paper

Subcellular calcium signaling in cardiac cells revealed with fast two-dimensional confocal imaging
Author(s): Lothar A. Blatter; Katherine A. Sheehan; Jens Kockskaemper
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Paper Abstract

Excitation-contraction (e-c) coupling in atrial myocytes was studied with fast confocal microscopy and simultaneous Ca2+-current measurements. Cat atrial myocytes lack transverse tubules and contain junctional (j-SR) and non- junctional SR (nj-SR) which both ave ryanodine receptor Ca2+ release channels. ICa triggered Ca2+ release from discrete peripheral j-SR release sites, which then fused into a peripheral 'ring' of elevated (Ca2+)i, followed by propagation to the center and contraction. J-SR Ca2+ release could be terminated instantaneously by interrupting ICa, whereas nj-SR Ca2+ release continued, once initiated, even after ICa and j-SR Ca2+ release was terminated. Resting myocytes exhibited spontaneous Ca2+ release events including Ca2+ release sites. In summary, during e-c coupling in atrial cells the elevation of (Ca2+)i begins with j-SR Ca2+ release under the tight control of ICa. The peripheral increase of (Ca2+)i subsequently activates adjacent nj-SR resulting in centripetal propagation of activation via CICR.

Paper Details

Date Published: 21 June 2002
PDF: 11 pages
Proc. SPIE 4626, Biomedical Nanotechnology Architectures and Applications, (21 June 2002); doi: 10.1117/12.472112
Show Author Affiliations
Lothar A. Blatter, Loyola Univ. (United States)
Katherine A. Sheehan, Loyola Univ. (United States)
Jens Kockskaemper, Loyola Univ. (United States)

Published in SPIE Proceedings Vol. 4626:
Biomedical Nanotechnology Architectures and Applications
Raymond P. Mariella; Michelle Palmer; Darryl J. Bornhop; Darryl J. Bornhop; Ramesh Raghavachari; Shuming Nie; Ramesh Raghavachari; Catherine J. Murphy; David A. Dunn; David A. Dunn; Raymond P. Mariella; Catherine J. Murphy; Dan V. Nicolau; Shuming Nie; Michelle Palmer; Ramesh Raghavachari, Editor(s)

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