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Proceedings Paper

Immobilized gold nanoparticle sensor for label-free optical detection of biomolecular interactions
Author(s): Nidhi Nath; Ashutosh Chilkoti
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Paper Abstract

We present a new label free optical technique to study biomolecular interactions in real time on a surface. This method monitors changes in the absorbance spectrum of a monolayer of gold on glass as a function of biomolecular binding. Gold nanoparticles with a diameter of 13 nm were chemisorbed onto an amine-terminated glass surface. The absorbance spectrum of the monolayer exhibited both a red shift as well as an increase in the absorbance at peak wavelength as a function of bulk solution refractive index. The increase in absorbance at peak wavelength as a function of bulk solution refractive index. The increase in absorbance at 550 nm was employed to study the kinetics of fibrinogen adsorption on the immobilized monolayer. The result obtained with the absorbance sensor were compared with those obtained using conventional SPR. This sensor is attractive because of its simplicity: gold nanoparticles are easily prepared with high reproducibility, they can be easily immobilized on glass, and their absorbance spectrum can be easily measured using widely available UV-vis spectrophotometers. Furthermore, this technique should be easily amenable to the design of chips in an array format for application in hgih-throughput immunoassays and proteomics.

Paper Details

Date Published: 21 June 2002
PDF: 8 pages
Proc. SPIE 4626, Biomedical Nanotechnology Architectures and Applications, (21 June 2002); doi: 10.1117/12.472110
Show Author Affiliations
Nidhi Nath, Duke Univ. (United States)
Ashutosh Chilkoti, Duke Univ. (United States)

Published in SPIE Proceedings Vol. 4626:
Biomedical Nanotechnology Architectures and Applications
Raymond P. Mariella; Michelle Palmer; Darryl J. Bornhop; Darryl J. Bornhop; Ramesh Raghavachari; Shuming Nie; Ramesh Raghavachari; Catherine J. Murphy; David A. Dunn; David A. Dunn; Raymond P. Mariella; Catherine J. Murphy; Dan V. Nicolau; Shuming Nie; Michelle Palmer; Ramesh Raghavachari, Editor(s)

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