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Proceedings Paper

AcycloPrime: a novel method for SNP analysis using fluorescence polarization
Author(s): Richard A. Greene; James J. DiMeo; Mary E. Malone; Suzanne Swartwout; Jianzhao Liu; Philip R. Buzby
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Paper Abstract

Single nucleotide polymorphisms (SNPs) are the most common type of genetic variation between individuals of a species and are therefore thought to be responsible for a large part of individual phenotypic variation. It has been estimated that a SNP may occur every 100-300 bases in the human genome. Research on human SNPs is expected to facilitate genetic mapping studies that may lead to a better understanding of the genetic basis for complex diseases and individual variation in drug metabolism. We have developed a novel assay for the identification of known SNPs using primer extension with the novel AcycloTerminators and a new thermostable polymerase, AcycloPol, in a homogeneous fluorescence polarization (FP) format. All assay steps can be performed in the same well of either a 384- or 96-well PCR-compatible microplate. FP provides several advantages, including simplicity and low reagent cost. The homogeneous assay format eliminates any need for separation or washing steps and is amenable to automation.

Paper Details

Date Published: 21 June 2002
PDF: 8 pages
Proc. SPIE 4626, Biomedical Nanotechnology Architectures and Applications, (21 June 2002); doi: 10.1117/12.472100
Show Author Affiliations
Richard A. Greene, PerkinElmer Life Sciences (United States)
James J. DiMeo, PerkinElmer Life Sciences (United States)
Mary E. Malone, PerkinElmer Life Sciences (United States)
Suzanne Swartwout, PerkinElmer Life Sciences (United States)
Jianzhao Liu, PerkinElmer Life Sciences (United States)
Philip R. Buzby, PerkinElmer Life Sciences (United States)


Published in SPIE Proceedings Vol. 4626:
Biomedical Nanotechnology Architectures and Applications
Raymond P. Mariella; Catherine J. Murphy; Michelle Palmer; David A. Dunn; Darryl J. Bornhop; David A. Dunn; Raymond P. Mariella; Catherine J. Murphy; Dan V. Nicolau; Shuming Nie; Michelle Palmer; Ramesh Raghavachari; Darryl J. Bornhop; Ramesh Raghavachari; Shuming Nie; Ramesh Raghavachari, Editor(s)

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