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Proceedings Paper

In vivo imaging of small animals with optical tomography and near-infrared fluorescent probes
Author(s): Matthew R. Palmer; Yasushi Shibata; Jonathan B. Kruskal; Robert E. Lenkinski
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Paper Abstract

A developmental optical tomography has been designed for imaging small animals in vivo using near IR fluorophores. The system employs epi-illumination via a 450 W Xe arc lamp, filtered and collimated to illuminate a 10 cm square movable stage. Emission light is filtered then collected by a high- resolution, high quantum efficiency, cooled CCD camera. Stage movement and image acquisition are under the control of a personal computer running system integration and automation software. During an experiment, the anesthetized animal is secured to the stage and up to 200 projections can be acquired over 180 degrees rotation. Angular sampling of the light distribution at a point on the surface is used to determine relative contributions form ballistic and diffuse photons. We have employed the system to investigate a number of applications of in-vivo fluorescent imaging. In dynamic studies, hepatic function has been visualized in nude mice following intravenous injection of indocyanine green (ICG) and cerebrospinal fluid flow as been measured by injection of ICG-lipoprotein conjugate in the subarachnoid space of the lumbar spine followed by dynamic imaging of the brain. Further applications in physiological imaging, cancer detection, and molecular imaging are under investigation in our laboratory.

Paper Details

Date Published: 21 June 2002
PDF: 4 pages
Proc. SPIE 4626, Biomedical Nanotechnology Architectures and Applications, (21 June 2002); doi: 10.1117/12.472078
Show Author Affiliations
Matthew R. Palmer, Beth Israel Deaconess Medical Ctr./Harvard Medical School (United States)
Yasushi Shibata, Beth Israel Deaconess Medical Ctr./Harvard Medical School (United States)
Jonathan B. Kruskal, Beth Israel Deaconess Medical Ctr./Harvard Medical School (United States)
Robert E. Lenkinski, Beth Israel Deaconess Medical Ctr./Harvard Medical School (United States)

Published in SPIE Proceedings Vol. 4626:
Biomedical Nanotechnology Architectures and Applications
Raymond P. Mariella; Catherine J. Murphy; Michelle Palmer; David A. Dunn; Darryl J. Bornhop; David A. Dunn; Raymond P. Mariella; Catherine J. Murphy; Dan V. Nicolau; Shuming Nie; Michelle Palmer; Ramesh Raghavachari; Darryl J. Bornhop; Ramesh Raghavachari; Shuming Nie; Ramesh Raghavachari, Editor(s)

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