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Proceedings Paper

Development of novel optically encoded microspheres for microarrays
Author(s): Vladimir A. Sabetsky; Serguei V. Martiouchine; Zinaida V. Nesterova
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Paper Abstract

The use of DNA microarrays for analysis of complex biological samples is becoming a mainstream part of biomedical research. Microarrays and gene chips containing a lot of specific polynucleotide sequence of both and novel, can be used to establish the expression of numerous genes in a single experiment. We developed a multicolor fluorescent microbeads for differential display method that combines properties of both differential signal amplification and DNA microarray in signal analysis. The multicolor fluorescent differential display provides a basis upon which a sensitive and accurate gene expression process can be automated and digitally analyzed. The ability to perform such analysis for extremely large nu8mbers of probes on an inexpensive and well-defined high-throughput platform is highly desirable. Microbead-based libraries are emerging as a very attractive alternative smaller probe sites. Most impressive advantages of microbeads are that they inexpensive to produce in a large numbers, they can be conveniently stored in a small volume of fluid, and they can be easily bar coded and screened using a variety of technologies. We developed a dextran-based microbeads containing covalently coupled fluorescent compounds. By combination of different compounds attached to dextran molecules it was possible to increase the number of 'fluorescently barcoded beads'.

Paper Details

Date Published: 21 June 2002
PDF: 4 pages
Proc. SPIE 4626, Biomedical Nanotechnology Architectures and Applications, (21 June 2002); doi: 10.1117/12.472065
Show Author Affiliations
Vladimir A. Sabetsky, Institute of Highly Pure Biopreparations (Russia)
Serguei V. Martiouchine, Institute of Highly Pure Biopreparations (Russia)
Zinaida V. Nesterova, Corning Scientific Ctr. (Russia)

Published in SPIE Proceedings Vol. 4626:
Biomedical Nanotechnology Architectures and Applications
Raymond P. Mariella; Catherine J. Murphy; Michelle Palmer; David A. Dunn; Darryl J. Bornhop; David A. Dunn; Raymond P. Mariella; Catherine J. Murphy; Dan V. Nicolau; Shuming Nie; Michelle Palmer; Ramesh Raghavachari; Darryl J. Bornhop; Ramesh Raghavachari; Shuming Nie; Ramesh Raghavachari, Editor(s)

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