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Proceedings Paper

Massively parallel microfluidics platform for storage and ultra-high-throughput screening
Author(s): Colin J. H. Brenan; Tom Morrison; Kristine Stone; Tara Heitner; Arrin Katz; Tanya S. Kanigan; Robert Hess; Soek-Jooh Kwon; Heung-Chae Jung; Jae-Gu Pan
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Paper Abstract

We have developed a novel microarray technology for performing very large numbers of biochemical, chemical and cell-based nanoliter volume synthesis, storage and screening operations in a massively parallel manner. The Living Chip is an array of precisely machined through-holes retaining nanoliters of fluid by capillary action. Sample loading, washing and recovery are operations that can be performed manually or with simple automation. Mixing between co- registered through-holes is achieved by stacking two or more precision aligned arrays and optical assay read-outs are in parallel with a CCD imaging system. An automated picker system transfers hits into lower density microtiter plates for further analysis. We will present result demonstrating massively parallel implementation of both homogeneous and inhomogeneous fluidic and cell-based assay systems and applications of the chip for drug compound library storage and management.

Paper Details

Date Published: 21 June 2002
PDF: 10 pages
Proc. SPIE 4626, Biomedical Nanotechnology Architectures and Applications, (21 June 2002); doi: 10.1117/12.472061
Show Author Affiliations
Colin J. H. Brenan, BioTrove, Inc. (United States)
Tom Morrison, BioTrove, Inc. (United States)
Kristine Stone, BioTrove, Inc. (United States)
Tara Heitner, BioTrove, Inc. (United States)
Arrin Katz, BioTrove, Inc. (United States)
Tanya S. Kanigan, BioTrove, Inc. (United States)
Robert Hess, BioTrove, Inc. (United States)
Soek-Jooh Kwon, GenoFocus, Inc. (South Korea)
Heung-Chae Jung, GenoFocus, Inc. (South Korea)
Jae-Gu Pan, GenoFocus, Inc. (South Korea)

Published in SPIE Proceedings Vol. 4626:
Biomedical Nanotechnology Architectures and Applications
Raymond P. Mariella; Michelle Palmer; Darryl J. Bornhop; Darryl J. Bornhop; Ramesh Raghavachari; Shuming Nie; Ramesh Raghavachari; Catherine J. Murphy; David A. Dunn; David A. Dunn; Raymond P. Mariella; Catherine J. Murphy; Dan V. Nicolau; Shuming Nie; Michelle Palmer; Ramesh Raghavachari, Editor(s)

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