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Proceedings Paper

Optical sensors for process monitoring in biotechnology
Author(s): F. Ploetz; Carsten Schelp; K. Anders; F. Eberhardt; Thomas-Helmut Scheper; F. Bueckmann
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Paper Abstract

The development and application of various optical sensors will be presented. Among these are optical sensors (optrodes) with immobilized enzymes, coenzymes, and labeled antibodies. The NADH formation of coenzyme dependent enzymes was used for detection of lactate, pyrovate mannitol, ethanol, and formate. An enzyme optrode based on a pH-optrode as a transducer for the monitoring of urea and penicillin in fermentation media was developed. For preparing an oxygen optrode, oxygen-sensitive fluorophores were entrapped in a gaspermeable silicone matrix that is attached to the distal end of a bifurcated fiber optic waveguide bundle. By labeling of immuncomponent with fluorophores or enzymes, which transpose fluorophores or chromophores, immunreactions were observed by an optical sensors.

Paper Details

Date Published: 1 September 1991
PDF: 7 pages
Proc. SPIE 1510, Chemical and Medical Sensors, (1 September 1991); doi: 10.1117/12.47142
Show Author Affiliations
F. Ploetz, Institut fuer Technische Chemie/Univ. of Hannover (Germany)
Carsten Schelp, Institut fuer Technische Chemie/Univ. of Hannover (Germany)
K. Anders, Institut fuer Technische Chemie/Univ. of Hannover (Germany)
F. Eberhardt, Institut fuer Technische Chemie/Univ. of Hannover (Germany)
Thomas-Helmut Scheper, Institut fuer Technische Chemie/Univ. of Hannover (Germany)
F. Bueckmann, GBF (Germany)


Published in SPIE Proceedings Vol. 1510:
Chemical and Medical Sensors
Otto S. Wolfbeis, Editor(s)

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