Share Email Print
cover

Proceedings Paper

Enantio-selective optrode for optical isomers of biologically active amines using a new lipophilic aromatic carrier
Author(s): Huarui He; Georg Uray; Otto S. Wolfbeis
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

This paper presents a method for optically sensing enantiomers (optical isomers) of biological amines such as norephedrine, and drugs such as the (Beta) -blocker propranolol. It is based on the use of a new lipophilic aromatic ammonium ion carrier (DODD) and a highly fluorescent lipophilic proton carrier (DZ 49) dissolved in a pvc membrane. Recognition of one of the enantiomers is accomplished by specific interaction of the amine with the optically active lipophilic substrate in a pvc membrane. The amine, which is present as an ammonium ion at physiological pH, is carried into the pvc membranes. Simultaneously, a proton is released from the proton carrier (a lipophilic xanthene dye) that thereby undergoes a change in both color and fluorescence intensity. The sensors respond to three analytes in the concentration range from 0.01 to 10 mM for propranolol, 0.3 to 100 mM for norephedrine, and 1 to 100 mM for 1-phenylethylamine. The selectivity coefficients (Kopt) are 0.8, 0.7, and 0.8 for propranolol, norephedrine,a nd 1-phenylethylamine, respectively. It is of potential utility for specifically recognizing one out of several isomers, in particular bioactive amines, where one form usually is active only. The carrier showed stronger affinity for compounds which contain naphthyl rather than phenyl substituents.

Paper Details

Date Published: 1 September 1991
PDF: 9 pages
Proc. SPIE 1510, Chemical and Medical Sensors, (1 September 1991); doi: 10.1117/12.47128
Show Author Affiliations
Huarui He, Karl-Franzens Univ. (Austria)
Georg Uray, Karl-Franzens Univ. (Austria)
Otto S. Wolfbeis, Karl-Franzens Univ. (Austria)


Published in SPIE Proceedings Vol. 1510:
Chemical and Medical Sensors
Otto S. Wolfbeis, Editor(s)

© SPIE. Terms of Use
Back to Top