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Proceedings Paper

Two-photon 3D mapping of tisssue endogenous fluorescence species based on fluorescence emission spectra
Author(s): Lily Laiho Hsu; Thomas M. Hancewicz; Peter D. Kaplan; Peter T. C. So
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Paper Abstract

Two-photon microscopy imaging of endogenous fluorescence has been shown to be a powerful method for the quantification of tissue structure and biochemistry. While autofluorescence is observed in many tissue types, the identities and distributions of these fluorophores have not been completely characterized. Image guided spectral analysis is being developed to aid in extracting spectroscopic components from two-photon images. This methodology is being applied to the study of human skin. In ex vivo specimens, the overall bulk emission spectrum of the skin, the layer-resolved emission spectra of the stratum corneum, stratum spinosum, basal layer, and dermis, and the emission spectra of surgically exposed dermis have been measured. From the image guided spectral analysis, it was determined that there are approximately five factors that contribute to most of the luminescence signals from human skin. The autofluorescent species identified include tryptophan, NAD(P)H, melanin (or localizing species), and elastin. The collagen matrix contributes to a second harmonic signal.

Paper Details

Date Published: 17 June 2002
PDF: 8 pages
Proc. SPIE 4620, Multiphoton Microscopy in the Biomedical Sciences II, (17 June 2002); doi: 10.1117/12.470702
Show Author Affiliations
Lily Laiho Hsu, Massachusetts Institute of Technology (United States)
Thomas M. Hancewicz, Unilever Research US, Inc. (United States)
Peter D. Kaplan, Unilever Research US, Inc. (United States)
Peter T. C. So, Massachusetts Institute of Technology (United States)

Published in SPIE Proceedings Vol. 4620:
Multiphoton Microscopy in the Biomedical Sciences II
Ammasi Periasamy; Peter T. C. So, Editor(s)

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