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Proceedings Paper

Demodulation signal processing in multiphoton imaging
Author(s): Walter G. Fisher; Eric A. Wachter; David W. Piston
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Paper Abstract

Multiphoton laser scanning microscopy offers numerous advantages, but sensitivity can be seriously affected by contamination from ambient room light. Typically, this forces experiments to be performed in an absolutely dark room. Since mode-locked lasers are used to generate detectable signals, signal-processing can be used to avoid such problems by taking advantage of the pulsed characteristics of such lasers. Demodulation of the fluorescence signal generated at the mode-locked frequency can result in significant reduction of interference from ambient noise sources. Such demodulation can be readily adapted to existing microscopes by inserting appropriate processor circuitry between the detector and data collection system, yielding a more robust microscope.

Paper Details

Date Published: 17 June 2002
PDF: 5 pages
Proc. SPIE 4620, Multiphoton Microscopy in the Biomedical Sciences II, (17 June 2002); doi: 10.1117/12.470700
Show Author Affiliations
Walter G. Fisher, Photogen, Inc. (United States)
Eric A. Wachter, Photogen, Inc. (United States)
David W. Piston, Vanderbilt Univ. Medical Ctr. (United States)


Published in SPIE Proceedings Vol. 4620:
Multiphoton Microscopy in the Biomedical Sciences II
Ammasi Periasamy; Peter T. C. So, Editor(s)

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