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Proceedings Paper

Multicolor two-photon scanning microscopy using a 16-channel photomultiplier
Author(s): Christof Buehler; Ki Hean Kim; Urs Greuter; Nick Schlumpf; Peter T. C. So
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Paper Abstract

Two-photon scanning microscopy has been successfully applied in studying tissue structures and biochemistry with subcellular spatial resolution. We developed a 16-channel two-photon scanning microscope with high-performance single photon counting readout electronics. The apparatus incorporates a mode-locked Ti:Sapphire laser, a scanning microscope, a spectrograph with multi-anode PMT (16 channels with ~ 7 to 10 nm spectral resolution each), and a 16-channel photon counting card (PhCC) with integrated high-speed data link (payload 1.2 Gbps). Each PhCC detection channel features single-photon sensitivity and 100 MHz photon counting bandwidth. The multi-color single-photon counting detection scheme allows ultra-sensitive measurements in a broad spectrum of biomedical applications. We present spectroscopic studies of ex vivo tissue autofluorescence.

Paper Details

Date Published: 17 June 2002
PDF: 14 pages
Proc. SPIE 4620, Multiphoton Microscopy in the Biomedical Sciences II, (17 June 2002); doi: 10.1117/12.470694
Show Author Affiliations
Christof Buehler, Paul Scherrer Institut (Switzerland)
Ki Hean Kim, Massachusetts Institute of Technology (United States)
Urs Greuter, Paul Scherrer Institut (Switzerland)
Nick Schlumpf, Paul Scherrer Institut (Switzerland)
Peter T. C. So, Massachusetts Institute of Technology (United States)

Published in SPIE Proceedings Vol. 4620:
Multiphoton Microscopy in the Biomedical Sciences II
Ammasi Periasamy; Peter T. C. So, Editor(s)

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