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Proceedings Paper

Functional imaging of photosensitizers using multiphoton microscopy
Author(s): Eric A. Wachter; Craig Dees; Jay Harkins; Walter G. Fisher; Timothy Scott
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Paper Abstract

Multiphoton imaging of the sub-cellular distribution of photosensitizers can provide important clues to their mechanism of action in tumors. We have used this tool to study distribution and pharmacology of photosensitizers in murine hepatoma tumor cells dosed with various photosensitizers. Upon photoactivation, Rose Bengal yields nearly immediate photolytic release of lysomal enzymes, resulting in catastrophic cell destruction within 5-30 minutes. Such marked response is quite different than that observed with other photosensitizer agents, and is consistent with in vivo studies illustrating that Rose Bengal is capable of causing extremely rapid destruction of treated tumors.

Paper Details

Date Published: 17 June 2002
PDF: 5 pages
Proc. SPIE 4620, Multiphoton Microscopy in the Biomedical Sciences II, (17 June 2002); doi: 10.1117/12.470688
Show Author Affiliations
Eric A. Wachter, Photogen, Inc. (United States)
Craig Dees, Photogen, Inc. (United States)
Jay Harkins, Photogen, Inc. (United States)
Walter G. Fisher, Photogen, Inc. (United States)
Timothy Scott, Photogen, Inc. (United States)

Published in SPIE Proceedings Vol. 4620:
Multiphoton Microscopy in the Biomedical Sciences II
Ammasi Periasamy; Peter T. C. So, Editor(s)

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