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Proceedings Paper

FRET imaging microscopy
Author(s): Brian Herman; Mao Sun; Atsushi Masuda; Hans C. Gerritsen; Victoria Frohlich Centonze
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Paper Abstract

Fluorescence Resonance Energy Transfer (FRET) Microscopy has been finding substantial utility in the measurement of a number of biological processes. Most microscopic techniques that have been developed to monitor FRET measure changes in the donor and acceptor emission or fluorescent lifetime of the donor. These include measurements of sensitized emission, acceptor photobleaching and fluorescent lifetime imaging (FLI). However, which of these approaches is the best for a given experimental situation and for use with multiphoton microscopy is not clear. Using mutant GFP FRET caspase-2 substrate targeted to mitochondria, we compare FRET efficiencies measured using sensitized emission, acceptor photobleaching and FLI.

Paper Details

Date Published: 17 June 2002
PDF: 10 pages
Proc. SPIE 4620, Multiphoton Microscopy in the Biomedical Sciences II, (17 June 2002); doi: 10.1117/12.470683
Show Author Affiliations
Brian Herman, Univ. of Texas Health Science Ctr. (United States)
Mao Sun, Texas Tech Medcial School (United States)
Atsushi Masuda, Univ. of Texas Health Science Ctr. (United States)
Hans C. Gerritsen, Univ. Utrecht (Netherlands)
Victoria Frohlich Centonze, Univ. of Texas Health Science Ctr. (United States)


Published in SPIE Proceedings Vol. 4620:
Multiphoton Microscopy in the Biomedical Sciences II
Ammasi Periasamy; Peter T. C. So, Editor(s)

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