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Proceedings Paper

Application of multiphoton steady state and lifetime imaging to mapping of tumor vascular architecture in vivo
Author(s): Simon Ameer-Beg; Paul R. Barber; R. J. Hodgkiss; R. J. Locke; Robert G. Newman; Gillian M. Tozer; Borivoj Vojnovic; J. Wilson
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Paper Abstract

Recent interest in vascular targeting and anti-angiogenic drug treatments for cancer has stimulated fundamental research regarding the modes of action of these drugs as well as studies of the development and re-modeling of the vascular network following treatment. Multiphoton fluorescence microscopy is employed for in vivo mapping of three-dimensional blood vessel distribution in tumors grown in rodent dorsal skin-flap window chamber preparations. Accurate visualization of the vasculature in three-dimensions allows us to perform dynamic experiments in thick biological specimens in vivo. Examples of in vivo imaging of tumor vasculature are given and compared to normal tissue vasculature. The dynamic responses of blood vessels to treatment with the vascular targeting drug combretastatin A4-P are presented and discussed. The implementation of time-domain imaging by reversed stop-start time-correlated single photon counting (RSS-TCSPC) is discussed as a method for feature extraction in the presence of exogenous and endogenous fluorophores. In particular, the segmentation of the vascular network is demonstrated. Additional contrast, indicative of probe environmental factors, may also be realized. We present examples of in vivo lifetime imaging as a method to elucidate the physiological processes of the tumor microenvironment.

Paper Details

Date Published: 17 June 2002
PDF: 11 pages
Proc. SPIE 4620, Multiphoton Microscopy in the Biomedical Sciences II, (17 June 2002); doi: 10.1117/12.470681
Show Author Affiliations
Simon Ameer-Beg, Mount Vernon Hospital (United Kingdom)
Paul R. Barber, Mount Vernon Hospital (United Kingdom)
R. J. Hodgkiss, Mount Vernon Hospital (United Kingdom)
R. J. Locke, Mount Vernon Hospital (United Kingdom)
Robert G. Newman, Mount Vernon Hospital (United Kingdom)
Gillian M. Tozer, Mount Vernon Hospital (United Kingdom)
Borivoj Vojnovic, Mount Vernon Hospital (United Kingdom)
J. Wilson, Mount Vernon Hospital (United Kingdom)


Published in SPIE Proceedings Vol. 4620:
Multiphoton Microscopy in the Biomedical Sciences II
Ammasi Periasamy; Peter T. C. So, Editor(s)

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