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Proceedings Paper

Characterization of two-photon point-spread-function in skin imaging applications
Author(s): Chen-Yuan Dong; Betty Yu; Lily Laiho Hsu; Peter T. C. So
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Paper Abstract

The development of two-photon fluorescence microscopy pushed optical microscopy into a new era. A major advantage of two-photon microscopy is the enhanced depth penetration achievable using near-infrared excitation. This unique feature enables imaging of physiological specimens often to the limit of working distances of high numerical aperture objectives. With the unprecedented capability for in-depth examination of physiological specimens, optical properties of the specimens can affect the imaging formation deep inside the samples. In this work, we address this issue by performing imaging of skin structures by two-photon microscopy. In particular, we like to address the issue of whether objectives with different immersion fluids can influence image parameters. In addition, we will discuss the various methodologies in which the point-spread-function (PSF) may be determined in the skin. Our conclusion is that a direct PSF measurement inside the skin is needed to fully characterize two-photon imaging parameters for optimization of two-photon skin imaging.

Paper Details

Date Published: 17 June 2002
PDF: 8 pages
Proc. SPIE 4620, Multiphoton Microscopy in the Biomedical Sciences II, (17 June 2002); doi: 10.1117/12.470680
Show Author Affiliations
Chen-Yuan Dong, National Taiwan Univ. (Taiwan)
Betty Yu, Massachusetts Institute of Technology (United States)
Lily Laiho Hsu, Massachusetts Institute of Technology (United States)
Peter T. C. So, Massachusetts Institute of Technology (United States)

Published in SPIE Proceedings Vol. 4620:
Multiphoton Microscopy in the Biomedical Sciences II
Ammasi Periasamy; Peter T. C. So, Editor(s)

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