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Proceedings Paper

Multiwavelength TCSPC lifetime imaging
Author(s): Wolfgang Becker; Axel Bergmann; Christoph Biskup; Thomas Zimmer; Nikolaj Kloecker; Klaus Benndorf
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Paper Abstract

We present a novel time-correlated single photon counting (TCPSC) imaging technique that allows time-resolved multi-wavelength imaging in conjunction with a laser scanning microscope and a pulsed excitation source. The technique is based on a four-dimensional histogramming process that records the photon density over time, the x-y coordinates of the scanning area and the detector channel number. The histogramming process avoids any time gating or wavelength scanning and therefore yields a near-perfect counting efficiency. Applied to resonance energy transfer (RET) measurements, the setup is capable to record time-resolved fluorescence decays for the donor and the acceptor simultaneously.

Paper Details

Date Published: 17 June 2002
PDF: 6 pages
Proc. SPIE 4620, Multiphoton Microscopy in the Biomedical Sciences II, (17 June 2002); doi: 10.1117/12.470679
Show Author Affiliations
Wolfgang Becker, Becker and Hickl GmbH (Germany)
Axel Bergmann, Becker and Hickl GmbH (Germany)
Christoph Biskup, Friedrich-Schiller-Univ. Jena (Germany)
Thomas Zimmer, Friedrich-Schiller-Univ. Jena (Germany)
Nikolaj Kloecker, Eberhard-Karls-Univ. Tubingen (Germany)
Klaus Benndorf, Friedrich-Schiller-Univ. Jena (Germany)

Published in SPIE Proceedings Vol. 4620:
Multiphoton Microscopy in the Biomedical Sciences II
Ammasi Periasamy; Peter T. C. So, Editor(s)

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