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Proceedings Paper

Cell dynamics with a novel phase referenced low-coherence interferometer with sub-wavelength and sub-hertz sensitivity
Author(s): Changhuei Yang; Adam Wax; Kamran Badizadegan; Ramachandra R. Dasari; Michael S. Feld
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Paper Abstract

We report the use of a highly sensitive phase based motion measurement technique to study the correlation of cellular metabolic rate with cellular motions. The technique is based on a modified Michelson interferometer with a composite laser beam of 1550 nm low coherence light and 775 nm CW light. In this system, motional artifacts from vibrations in the interferometer are completely eliminated. We demonstrate that the system is sensitive to motions as small as 3.6 nm and velocities as small as 1 nm/s. Using the system, we show that the cellular motions are strongly dependent on the ambient temperature. We observe that the dependency does not conform to Brownian motion predictions but instead appears to correlate with the optical ambient temperature that the cells have evolved to operate in.

Paper Details

Date Published: 14 June 2002
PDF: 8 pages
Proc. SPIE 4619, Coherence Domain Optical Methods in Biomedical Science and Clinical Applications VI, (14 June 2002); doi: 10.1117/12.470484
Show Author Affiliations
Changhuei Yang, Massachusetts Institute of Technology (United States)
Adam Wax, Massachusetts Institute of Technology (United States)
Kamran Badizadegan, Massachusetts Institute of Technology (United States)
Ramachandra R. Dasari, Massachusetts Institute of Technology (United States)
Michael S. Feld, Massachusetts Institute of Technology (United States)


Published in SPIE Proceedings Vol. 4619:
Coherence Domain Optical Methods in Biomedical Science and Clinical Applications VI
Valery V. Tuchin; Joseph A. Izatt; James G. Fujimoto, Editor(s)

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