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Proceedings Paper

Comparative OCT imaging of the human esophagus: How well can we localize the muscularis mucosae?
Author(s): Inci F. Cilesiz; Paul Fockens; Raphaela P.A. Kerindongo; Dirk J. Faber; Guido N. J. Tytgat; Febo ten Kate; Ton G. J. M. van Leeuwen
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Paper Abstract

Early diagnosis with esophageal cancer limited to the mucosa will allow for local endoscopic treatment and improve prognosis. We compared with histology OCT images of healthy human esophageal tissue from two systems operating at 800 and 1275 nm to investigate which wavelength was best suited for detailed OCT imaging of the esophageal wall, and to localize the muscularis mucosae. Within an hour of surgical resection, an esophageal specimen was cleaned of excess blood and soaked in formalin for a minimum of 48 hours. In order to precisely localize the different layers of the esophageal wall on an OCT image, well-defined structures within the esophageal wall were sought. Following OCT imaging the specimen was prepared for routine histology. We observed that our 1275 nm system with 12 micrometers resolution was superior in terms of penetration. As compared to histology, the 4 micrometers resolution of our 800 nm system made fine details more visible. Using either system, a minimally trained eye could recognize the muscularis mucosae as a hypo-reflective layer. Although different conditions may apply in vivo, our ex vivo study paves the path to precise interpretation of OCT images of the esophageal wall.

Paper Details

Date Published: 14 June 2002
PDF: 7 pages
Proc. SPIE 4619, Coherence Domain Optical Methods in Biomedical Science and Clinical Applications VI, (14 June 2002); doi: 10.1117/12.470482
Show Author Affiliations
Inci F. Cilesiz, Univ. of Amsterdam/Academic Medical Ctr. (Turkey)
Paul Fockens, Univ. of Amsterdam/Academic Medical Ctr. (Netherlands)
Raphaela P.A. Kerindongo, Univ. of Amsterdam/Academic Medical Ctr. (Netherlands)
Dirk J. Faber, Univ. of Amsterdam/Academic Medical Ctr. (Netherlands)
Guido N. J. Tytgat, Univ. of Amsterdam/Academic Medical Ctr. (Netherlands)
Febo ten Kate, Univ. of Amsterdam/Academic Medical Ctr. (Netherlands)
Ton G. J. M. van Leeuwen, Univ. of Amsterdam/Academic Medical Ctr. (Netherlands)


Published in SPIE Proceedings Vol. 4619:
Coherence Domain Optical Methods in Biomedical Science and Clinical Applications VI
Valery V. Tuchin; Joseph A. Izatt; James G. Fujimoto, Editor(s)

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