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Proceedings Paper

Photothermal response of live cell depends upon cell metabolic state
Author(s): Dmitry Lapotko; Tat'yana Romanovskaya; Elena Gordiyko
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Paper Abstract

Photoacoustic and photothermal (PT) methods in live cells studies allow to realize direct monitoring as they do not require cell pretreatment with chemicals and because the sources of output signal are cell chromophores. The origin of PT signals from single cells that are irradiated with short pump laser pulse was studied experimentally under different rates of cell metabolism. The cells in different metabolic status were found to produce PT response of specific shape that occurs in specific pump energy region for each pump wavelength. This type of PT response is associated with secondary phenomena that are induced in cells by thermal impact. An ability of live cell to produce such PT response was found to depend upon the redox state of cell respiratory chain (RC) components that absorb pump laser radiation. The results of PT studies of in vitro modulation of hepatocyte metabolic state are presented as function of RC state (reduced/oxidized) and laser parameters (wavelength and energy). Obtained results show that several parameters of laser-cell interaction depend upon RC redox state: shape and amplitude of PT response from single cell, energy threshold for laser damage to cell.

Paper Details

Date Published: 10 June 2002
PDF: 8 pages
Proc. SPIE 4618, Biomedical Optoacoustics III, (10 June 2002); doi: 10.1117/12.469854
Show Author Affiliations
Dmitry Lapotko, Luikov Heat and Mass Transfer Institute (United States)
Tat'yana Romanovskaya, Luikov Heat and Mass Transfer Institute (Belarus)
Elena Gordiyko, Institute of Photobiology (Belarus)

Published in SPIE Proceedings Vol. 4618:
Biomedical Optoacoustics III
Alexander A. Oraevsky, Editor(s)

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