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Proceedings Paper

Multiparametric analysis of fine needle aspirate biopsies from parotid tumors by laser scanning cytometry (LSC)
Author(s): Andreas O.H. Gerstner; Julia Machlitt; Anne-Kathrin Mueller; Attila Tarnok; Jens Oeken; Friedrich Bootz
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Paper Abstract

In order to minimize hospitalization and morbidity with optimized therapy for a patient with a tumor of the parotid gland a malignancy must be confirmed or excluded as soon as possible. Up to now, non- and minimal-invasive methods do not yield this information. For fine needle aspirate biopsies (FNABs), analysis by a specialized cytologist yields subjective and qualitative but not objective and quantitative data. LSC is a semi-automated microscope-based technology and offers ideal prerequisites for the analysis of specimens fixed on a slide. We have established an assay for FNABs from parotid gland tumors. Cells are stained for cytokeratin and DNA. The analysis quantitatively determines the ploidy of the cells and the degree of condensation of the DNA; on this basis the percentage of cells undergoing mitosis can be determined. Subsequently the cells are stained by H&E and are re-localized on the slide at their fixed position. Micrographs are taken for objective documentation of the cells' morphology. Using this assay FNABs from parotid gland tumors were analyzed; tumors that were diagnosed as benign by routine histopathology showed no aneuploidy whereas malignant tumors were aneuploid. This preliminary study demonstrates the capacities of LSC for minimal-invasive assays yielding quantitative and objective data.

Paper Details

Date Published: 4 June 2002
PDF: 11 pages
Proc. SPIE 4625, Clinical Diagnostic Systems: Technologies and Instrumentation, (4 June 2002); doi: 10.1117/12.469791
Show Author Affiliations
Andreas O.H. Gerstner, Univ. of Leipzig (Germany)
Julia Machlitt, Univ. of Leipzig (Germany)
Anne-Kathrin Mueller, Univ. of Leipzig (Germany)
Attila Tarnok, Univ. of Leipzig (Germany)
Jens Oeken, Univ. of Leipzig (Germany)
Friedrich Bootz, Univ. of Leipzig (Germany)

Published in SPIE Proceedings Vol. 4625:
Clinical Diagnostic Systems: Technologies and Instrumentation
Gerald E. Cohn, Editor(s)

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