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Proceedings Paper

Yeast and mammalian metabolism continuous monitoring by using pressure recording as an assessment technique for xenobiotic agent effects
Author(s): Marziale Milani; Monica Ballerini; Lorenzo Ferraro; E. Marelli; Francesca Mazza; Matteo Zabeo
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Paper Abstract

Our work is devoted to the study of Saccharomyces cerevisiae and human lymphocytes cellular metabolism in order to develop a reference model to assess biological systems responses to chemical or physical agents exposure. CO2 variations inside test-tubes are measured by differential pressure sensors; pressure values are subsequently converted in voltage. The system allows to test up to 16 samples at the same time. Sampling manages up to 100 acquisitions per second. Values are recorded by a data acquisition card connected to a computer. This procedure leads to a standard curve (pressure variation versus time), typical of the cellular line, that describe cellular metabolism. The longest time lapse used is of 170 h. Different phases appear in this curve: an initial growth up to a maximum, followed by a decrement that leads to a typical depression (pressure value inside the test-tubes is lower than the initial one) after about 35 h from the beginning of yeast cells. The curve is reproducible within an experimental error of 4%. The analysis of many samples and the low cost of the devices allow a good statistical significance of the data. In particular as a test we will compare two sterilizing agents effects: UV radiation and amuchina.

Paper Details

Date Published: 4 June 2002
PDF: 14 pages
Proc. SPIE 4625, Clinical Diagnostic Systems: Technologies and Instrumentation, (4 June 2002); doi: 10.1117/12.469775
Show Author Affiliations
Marziale Milani, INFM and Univ. degli Studi di Milano-Bicocca (Italy)
Monica Ballerini, Univ. degli Studi di Milano-Bicocca (Italy)
Lorenzo Ferraro, Univ. degli Studi di Milano-Bicocca (Italy)
E. Marelli, Univ. degli Studi di Milano-Bicocca (Italy)
Francesca Mazza, Univ. degli Studi di Milano-Bicocca (Italy)
Matteo Zabeo, Univ. degli Studi di Milano-Bicocca (Italy)

Published in SPIE Proceedings Vol. 4625:
Clinical Diagnostic Systems: Technologies and Instrumentation
Gerald E. Cohn, Editor(s)

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