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Proceedings Paper

Knowledge-based image processing for on-off type DNA microarray
Author(s): Jong Dae Kim; Seo Kyu Kim; Jeong Sik Cho; Jongwon Kim
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Paper Abstract

This paper addresses the image processing technique for discriminating whether the probes are hybrized with target DNA in the Human Papilloma Virus (HPV) DNA Chip designed for genotyping HPV. In addition to the probes, the HPV DNA chip has markers that always react with the sample DNA. The positions of probe-dots in the final scanned image are fixed relative to the marker-dot locations with a small variation according to the accuracy of the dotter and the scanner. The probes are duplicated 4 times for the diagnostic stability. The prior knowledges such as the maker relative distance and the duplication information of probes is integrated into the template matching technique with the normalized correlation measure. Results show that the employment of both of the prior knowledges is to simply average the template matching measures over the positions of the markers and probes. The eventual proposed scheme yields stable marker locating and probe classification.

Paper Details

Date Published: 5 June 2002
PDF: 9 pages
Proc. SPIE 4623, Functional Monitoring and Drug-Tissue Interaction, (5 June 2002); doi: 10.1117/12.469458
Show Author Affiliations
Jong Dae Kim, Hallym Univ. (South Korea)
Seo Kyu Kim, Biomedisys Co., Inc. (South Korea)
Jeong Sik Cho, Biomedisys Co., Ltd. (South Korea)
Jongwon Kim, Biomedlab Co., Ltd. (South Korea)

Published in SPIE Proceedings Vol. 4623:
Functional Monitoring and Drug-Tissue Interaction
Manfred D. Kessler; Gerhard J. Mueller, Editor(s)

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