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Proceedings Paper

Hypokinesia of myocardium of perfused rat heart at different oxygenation of myoglobin and redox state of cytochrome
Author(s): Klaus H. Frank; J. Zuendorf; D. Tauschek; Manfred D. Kessler
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Paper Abstract

Questions about development of hypo-kinetic zones in myocardium of patients suffering from severe coronary heart disease are discussed controversially among heart surgeons. We established a model for isolated and hemoglobin free perfusion of rat heart in which sufficient flow was established within all capillaries and thus existence of ischemic capillaries could be excluded. A definite diagnosis of tissue anoxia is only possible by optical measurements of the oxidation and the reduction (redox state) of the cytochrome oxidase of intact myocytes. Therefore, we used an EMPHO for this kind of measurements. Intracellular oxygenation of myoglobin oxygenation (MbO2) and redox state of cytochrome aa3, b and c were recorded in the outer wall of working, hypo-kinetic and a-kinetic myocardium. As a result of our investigations we were able to prove that by lowering at the venous end of capillaries tissue pO2 and myoglobin oxygenation stepwise below 5 mmHg and 50% of saturation respectively, a continuous decrease of myocardial contractility could be achieved.

Paper Details

Date Published: 5 June 2002
PDF: 8 pages
Proc. SPIE 4623, Functional Monitoring and Drug-Tissue Interaction, (5 June 2002); doi: 10.1117/12.469455
Show Author Affiliations
Klaus H. Frank, Dornier MedTech America Inc. (United States)
J. Zuendorf, Friedrich-Alexander-Univ. Erlangen-Nuernberg (Germany)
D. Tauschek, Friedrich-Alexander-Univ. Erlangen-Nuernberg (Germany)
Manfred D. Kessler, Friedrich-Alexander-Univ. Erlangen-Nuernberg (Germany)

Published in SPIE Proceedings Vol. 4623:
Functional Monitoring and Drug-Tissue Interaction
Manfred D. Kessler; Gerhard J. Mueller, Editor(s)

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