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Proceedings Paper

Intravital fluorescence microscopic study of the behavior of long-circulating liposomes during microvascular thrombosis
Author(s): Jean-Marie Dvoisselle; Sylvie Begu; Corine Tourne-Peteilh; Bruno Buys; Serge R. Mordon
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Paper Abstract

Treatment of thrombosis depends on the selectivity of thrombolytic agents to the clot. It has been already demonstrated that liposomes can provide a better selectivity of such agents to the clot site. We have recently shown that intravital fluorescence microscopy is a powerful tool to image in situ and in real time the labeling of leukocytes by long circulating liposomes. The aim of this study was to monitor the in vivo behavior of such liposomes in a clot site. Carboxyfluorescein-loaded long circulating liposomes were prepared and characterized in term of size and permeability. The liposomes suspension was injected intravenously to golden hamsters. The skin microcirculation was observed using a dorsal skin-fold chamber by fluorescence microscopy. Thrombosis were obtained as the consequence of the inflammatory response due to the surgery. Using this model, fluorescent dots were observed at the site of the clot. Liposomes accumulate at the clot site whatever the mechanism (passive deposition or uptake). There is a period of latency and 30 seconds after the blood flow stop, fluorescence increases very rapidly and a bright fluorescent spot is observed at the site of the clot. Further studies are needed to determine the exact localization of liposomes in the clot and the mechanism of interaction.

Paper Details

Date Published: 5 June 2002
PDF: 8 pages
Proc. SPIE 4623, Functional Monitoring and Drug-Tissue Interaction, (5 June 2002); doi: 10.1117/12.469445
Show Author Affiliations
Jean-Marie Dvoisselle, CNRS and Univ. of Montpellier I (France)
Sylvie Begu, CNRS and Univ. de Montpellier I (France)
Corine Tourne-Peteilh, CNRS and Univ. de Montpellier I (France)
Bruno Buys, INSERM (France)
Serge R. Mordon, INSERM (France)

Published in SPIE Proceedings Vol. 4623:
Functional Monitoring and Drug-Tissue Interaction
Manfred D. Kessler; Gerhard J. Mueller, Editor(s)

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