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Proceedings Paper

Targeted photodynamic therapy for infected wounds in mice
Author(s): Michael R. Hamblin; David A. O'Donnell; Touqir Zahra; Christopher H. Contag; Albert T. McManus; Tayyaba Hasan
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Paper Abstract

Although many workers have used photodynamic therapy to kill bacteria in vitro, the use of this approach has seldom been reported in vivo in animal models of infection. We report on the use of a targeted polycationic photosensitizer conjugate between poly-L-lysine and chlorin(e6) that can penetrate the Gram (-) outer membrane together with red laser light to kill Escherichia coli and Pseudomonas aeruginosa infecting excisional wounds in mice. We used genetically engineered luminescent bacteria that allowed the infection to be imaged in mouse wounds using a sensitive CCD camera. Wounds were infected with 5x106 bacteria, followed by application of the conjugate in solution and illumination. There was a light-dose dependent loss of luminescence as measured by image analysis in the wound treated with conjugate and light, not seen in control wounds. This strain of E coli is non-invasive and the infection in untreated wounds spontaneously resolved in a few days and all wounds healed equally well showing the photodynamic treatment did not damage the host tissue. P aeruginosa is highly invasive and mice with untreated or control wounds all died while 90% of PDT treated mice survived. PDT may have a role to play in the rapid treatment of infected wounds in view of the worldwide rise in antibiotic resistance.

Paper Details

Date Published: 6 June 2002
PDF: 11 pages
Proc. SPIE 4612, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XI, (6 June 2002); doi: 10.1117/12.469354
Show Author Affiliations
Michael R. Hamblin, Wellman Labs. of Photomedicine/Massachusetts General Hospital and Harvard Medical School a (United States)
David A. O'Donnell, Wellman Labs. of Photomedicine/Massachusetts General Hospital (United States)
Touqir Zahra, Wellman Labs. of Photomedicine/Massachusetts General Hospital and Harvard Medical School a (United States)
Christopher H. Contag, Stanford Univ. School of Medicine (United States)
Albert T. McManus, U.S. Army Institute of Surgical Research (United States)
Tayyaba Hasan, Wellman Labs. of Photomedicine/Massachusetts General Hospital and Harvard Medical School a (United States)


Published in SPIE Proceedings Vol. 4612:
Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XI
Thomas J. Dougherty, Editor(s)

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