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Proceedings Paper

Effects of low-fluence-rate PDT on glioma spheroids
Author(s): Steen J. Madsen; Robin M. Rodenbush; Chung-Ho Sun; Bruce J. Tromberg; Henry Hirschberg
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Paper Abstract

The effectiveness of low-fluence rate PDT on human glioma spheroids incubated in 5-aminolevulinic acid (ALA) or hexyl- ALA, is investigated. Spheroid survival is monitored following PDT at representative drug concentrations, light fluences and fluence rates. It is shown that, for above threshold fluences, significant PDT response can be obtained at a fluence rate of 5 mW cm-2. At this fluence rate, the response of human glioma spheroids to PDT with lipophilic ester derivatives, such as hexyl-ALA, is equivalent to that obtained with ALA, however, this equivalency is observed for ester concentrations 20 times lower than the parent compound. The efficacy of PDT is significantly reduced at a sub-threshold fluence of 25 J cm-2. Spheroid response appears to be somewhat fluence rate dependent at sub-threshold fluences - survival is marginally poorer at the higher fluence rate (25 mW cm-2) investigated. In addition, the efficacy of PDT at 25 J cm-2 depends significantly on photosensitizer concentration at the lowest fluence rate investigated (5 mW cm-2).

Paper Details

Date Published: 6 June 2002
PDF: 6 pages
Proc. SPIE 4612, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XI, (6 June 2002); doi: 10.1117/12.469347
Show Author Affiliations
Steen J. Madsen, Univ. of Nevada/Las Vegas and Beckman Laser Institute and Medical Ctr. (United States)
Robin M. Rodenbush, Univ. of Nevada/Las Vegas (United States)
Chung-Ho Sun, Beckman Laser Institute and Medical Clinic (United States)
Bruce J. Tromberg, Beckman Laser Institute and Medical Clinic (United States)
Henry Hirschberg, Rikshospitalet and Beckman Laser Institute and Medical Clinic (Norway)


Published in SPIE Proceedings Vol. 4612:
Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XI
Thomas J. Dougherty, Editor(s)

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