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Proceedings Paper

Measurement of singlet oxygen luminescence from AML5 cells sensitized with ALA-induced PpIX in suspension during photodynamic therapy and correlation with cell viability after treatment
Author(s): Mark J. Niedre; Michael S. Patterson; Natalie Boruvka; Brian C. Wilson
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Paper Abstract

Photodynamic therapy (PDT) treatments were performed on suspensions of AML5 cells incubated with ALA-induced PpIX with either 15 mW/cm2, 45 mW/cm2 or 90 mW/cm2 pulsed 523 nm light. Singlet oxygen (1O2) luminescence measurements were carried out using a detection system including a near-infrared (NIR) sensitive photomultiplier tube (PMT). Cell viability was determined at several time points during treatments using the propidium iodide (PI) flow cytometry assay. Cumulative 1O2 measurements were compared to cell viability for all experiments. Preliminary results show that average cell viability increased with increasing treatment irradiance. Cell viability was found to correlate well with absolute cumulative 1O2 luminescence measurements during treatment.

Paper Details

Date Published: 6 June 2002
PDF: 9 pages
Proc. SPIE 4612, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XI, (6 June 2002); doi: 10.1117/12.469336
Show Author Affiliations
Mark J. Niedre, Ontario Cancer Institute/Princess Margeret Hospital and Univ. of Toronto (Canada)
Michael S. Patterson, Hamilton Regional Cancer Ctr./McMaster Univ. (Canada)
Natalie Boruvka, Ontario Cancer Institute/Princess Margeret Hospital and Univ. of Toronto (Canada)
Brian C. Wilson, Ontario Cancer Institute/Princess Margeret Hospital and Univ. of Toronto (Canada)


Published in SPIE Proceedings Vol. 4612:
Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XI
Thomas J. Dougherty, Editor(s)

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