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Proceedings Paper

Antimicrobial effect of low-level laser therapy in the presence of photosensitizer on human saliva bacteria
Author(s): Iriana Carla J. Zanin; Aldo Brugnera; Jose Francisco Hoefling; Fatima A. A. Zanin; Reginaldo Bruno Goncalves
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Paper Abstract

The purpose of this in vitro study was to determine whether low-level laser light in the presence of a photosensitizer can kill streptococci in human saliva. Samples of stimulated saliva were collected from ten healthy subjects. These samples were mixed and exposed to a gallium-aluminium-arsenide laser light (660 nm) in the presence of toluidine blue O. Viable total streptococci and mutans streptococci were counted on plates with MSA and MSB, respectively, following incubation at 37 degree(s)C, 10% CO2 for 48 hours. The exposure of saliva to laser light in the absence of the dye, or the dye in the absence of the laser light showed no significant effect on the viability of the microorganisms. A decrease in the number of viable microorganisms was only verified when they were exposed to both the laser light and the dye at the same time. A total inhibition growth of the mutans streptococci and a substantial killing rate of total streptococci were achieved with a dye concentration of 100 mg/mL and an energy density of 28.8 J/cm2. These results imply that total streptococci and mutans streptococci present in human saliva can be killed by low-level laser light in the presence of toluidine blue O.

Paper Details

Date Published: 3 June 2002
PDF: 8 pages
Proc. SPIE 4610, Lasers in Dentistry VIII, (3 June 2002); doi: 10.1117/12.469319
Show Author Affiliations
Iriana Carla J. Zanin, Univ. Estadual de Campinas (Brazil)
Aldo Brugnera, Univ. Federal do Rio de Janeiro, Univ. Camilo Castelo Branco, and APCD (Brazil)
Jose Francisco Hoefling, Univ. Estadual de Campinas (Brazil)
Fatima A. A. Zanin, Univ. Federal do Rio de Janeiro, Univ. Camilo Castelo Branco, and APCD (Brazil)
Reginaldo Bruno Goncalves, Univ. Estadual de Campinas (Brazil)


Published in SPIE Proceedings Vol. 4610:
Lasers in Dentistry VIII
Peter Rechmann; Daniel Fried; Thomas Hennig, Editor(s)

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