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Proceedings Paper

In-vitro study of bactericidal effect of low-level laser therapy in the presence of photosensitizer on cariogenic bacteria
Author(s): Iriana Carla J. Zanin; Aldo Brugnera; Reginaldo Bruno Goncalves
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Paper Abstract

The aim of this in vitro study was to determine whether low-level laser light in the presence of a photosensitizer could kill Streptococcus mutans and Streptococcus sobrinus. Suspensions of these microorganisms were exposed to a gallium-aluminium-arsenide laser light (660 nm) in the presence of photosensitizer toluidine blue O. Viable microorganisms were counted on brain heart agar plates after incubation at 37 degree(s)C in partial atmosphere of 10% CO2 for 48 hours. Their exposure to the laser light in the absence of the dye or the dye in the absence of the laser light presented no significant effect on the viability of the microorganisms. However, a decrease in the number of viable microorganisms was only verified when they were exposed to both the laser light and the dye at the same time. Their total growth inhibition was achieved with a dye concentration of 100 mg/mL and a light energy density of 28.8 J/cm2, after being exposed to laser light for 900 seconds. In conclusion, these results imply that these bacteria can be killed by low-power laser light in the presence of the photosensitizer.

Paper Details

Date Published: 3 June 2002
PDF: 8 pages
Proc. SPIE 4610, Lasers in Dentistry VIII, (3 June 2002); doi: 10.1117/12.469318
Show Author Affiliations
Iriana Carla J. Zanin, Univ. Estadual de Campinas (Brazil)
Aldo Brugnera, Univ. Federal do Rio de Janeiro, Univ. Camilo Castelo Branco, and APCD (Brazil)
Reginaldo Bruno Goncalves, Univ. Estadual de Campinas (Brazil)

Published in SPIE Proceedings Vol. 4610:
Lasers in Dentistry VIII
Peter Rechmann; Daniel Fried; Thomas Hennig, Editor(s)

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