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Proceedings Paper

Resolution limits and noise reduction in digital holographic microscopy
Author(s): Allan K. Evans
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Paper Abstract

In digital holographic microscopy, a hologram of an object is recorded by an electronic image sensors and a computer is used to reconstruct the original object numerically. A number ro different arrangements have been successfully used, for example by Haddad, Schnars, Takaki and Jacquot. There is an intermediate case between the Fourier-transform method of Haddad et al and the Fresnel arrangement used by Jacquot et al, which has some of the advantages of both methods. A point reference source in a lane some distance from object provides the spatial frequency reduction in the hologram plane, as for Fourier transform methods, without the strong central peak and with the twin image defocused in the object plane. This arrangement is tested, showing that it can produce a resolution significantly improved over the Fresnel case. We also consider the removal of the holographic twin image in the Fresnel in-line holographic arrangement. The high-contrast parts of the image are assumed to be part of the true image, and the twin image corresponding to these high-contrast elements is subtracted, leaving an improved estimate of the true image only. We present experimental results demonstrating this method for a number of different objects.

Paper Details

Date Published: 3 June 2002
PDF: 9 pages
Proc. SPIE 4659, Practical Holography XVI and Holographic Materials VIII, (3 June 2002); doi: 10.1117/12.469277
Show Author Affiliations
Allan K. Evans, De Montfort Univ. (United Kingdom)

Published in SPIE Proceedings Vol. 4659:
Practical Holography XVI and Holographic Materials VIII
Sylvia H. Stevenson; Stephen A. Benton; T. John Trout; Sylvia H. Stevenson; T. John Trout, Editor(s)

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