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Proceedings Paper

Visualization of intracellular Ca2+ dynamics with third-harmonic generation microscopy
Author(s): R. Barille; S. Rivet; Lionel Canioni; Laurent Sarger; Pierre Vacher; Pierre Voisin
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Paper Abstract

Measurements by Laser scanning Third Harmonic Generation microscopy of Ca2+ dynamic release from internal stores and/or calcium influx in biological cells is presented. The Ca2+ signal consists of a transient increase in the intracellular concentration. A good correlation is found between these measurements and measurements done with microspectrofluorometry.

Paper Details

Date Published: 30 May 2002
PDF: 7 pages
Proc. SPIE 4749, ICONO 2001: Novel Trends in Nonlinear Laser Spectroscopy and Optical Diagnostics and Lasers in Chemistry, Biophysics, and Biomedicine, (30 May 2002); doi: 10.1117/12.468897
Show Author Affiliations
R. Barille, Univ. Bordeaux I (France)
S. Rivet, Univ. Bordeaux I (France)
Lionel Canioni, Univ. Bordeaux I (France)
Laurent Sarger, Univ. Bordeaux I (France)
Pierre Vacher, CNRS (France)
Pierre Voisin, CNRS (France)


Published in SPIE Proceedings Vol. 4749:
ICONO 2001: Novel Trends in Nonlinear Laser Spectroscopy and Optical Diagnostics and Lasers in Chemistry, Biophysics, and Biomedicine

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