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Proceedings Paper

Theoretical investigation of the signal-to-noise ratio for different fluorescence lifetime imaging techniques
Author(s): Kjell Carlsson; Johan Philip
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Paper Abstract

Using Monte-Carlo methods, we have investigated the signal- to-noise ratio obtainable for different fluorescence lifetime imaging methods. Quantum noise limited performance and mono-exponential decays were assumed. We have also investigated the importance of parameter choice and implementation for the different methods. In addition, our simulations were in many cases compared with analytical theoretical investigations. The results from the simulations proved to be in good agreement with the theoretical results. It was found that all the investigated lifetime imaging methods have the potential to produce a high signal-to-noise ratio, but careful attention must be paid to implementation method and parameter choice in order to get optimal results.

Paper Details

Date Published: 28 May 2002
PDF: 9 pages
Proc. SPIE 4622, Optical Diagnostics of Living Cells V, (28 May 2002); doi: 10.1117/12.468362
Show Author Affiliations
Kjell Carlsson, Royal Institute of Technology (Sweden)
Johan Philip, Royal Institute of Technology (Sweden)

Published in SPIE Proceedings Vol. 4622:
Optical Diagnostics of Living Cells V
Robert C. Leif; Daniel L. Farkas; Robert C. Leif, Editor(s)

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