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Proceedings Paper

Delayed luminescence technique: a probe for vital processes in biological systems
Author(s): Monica Ballerini; Marziale Milani; Lorenzo Ferraro; Matteo Zabeo; Francesco Musumeci
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Paper Abstract

Delayed Luminescence is a well established technique based on the illumination of biological sample and on the subsequent count of the number of photons re-emitted by the sample after the light source has been switched off together with their spectral distribution. Investigations have been performed on yeast cells and algae so that correlations can be established between biological activities and physical parameters of the samples. Moreover nonlinear mechanisms of interaction between optical fields and cells can be figured out. Further investigations will be reported on yeast cell samples deposited on paper filters after irradiation by soft X-rays. The results will be discussed by cross correlating the experimental evidence from Delayed Luminescence with those obtained by metabolic activity recording. Luminescence and Delayed Luminescence are strictly correlated with early insurgence of morphological alterations of normal or pathological nature in cells and tissues; a novel technique for morphological analysis has been developed by means of Focused Ion Beam machines. A straightforward approach to morphology at the nanoscale both of membranes and cellular inner structures is then made possible. The final aim is an experimental set up for an early and reliable detection technique for neoplastic cells and tissues sorting.

Paper Details

Date Published: 28 May 2002
PDF: 10 pages
Proc. SPIE 4622, Optical Diagnostics of Living Cells V, (28 May 2002); doi: 10.1117/12.468351
Show Author Affiliations
Monica Ballerini, INFM and Univ. delgi Studi di Milano-Bicocca (Italy)
Marziale Milani, INFM and Univ. degli Studi di Milano-Bicocca (Italy)
Lorenzo Ferraro, Univ. delgi Studi di Milano-Bicocca (Italy)
Matteo Zabeo, Univ. delgi Studi di Milano-Bicocca (Italy)
Francesco Musumeci, Univ. degli Studi di Catania (Italy)

Published in SPIE Proceedings Vol. 4622:
Optical Diagnostics of Living Cells V
Robert C. Leif; Daniel L. Farkas; Robert C. Leif, Editor(s)

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