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Proceedings Paper

Imaging microscopic fluid viscosity and velocity fields using confocal scanning optical tweezers
Author(s): Boaz A. Nemet; Nili Harnik; Mark Cronin-Golomb
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Paper Abstract

Confocal microscopy and optical tweezers were combined to develop a minimally invasive instrument capable of making hydrodynamic measurements more rapidly than is possible with other devices. This result leads to the possibility of making scanning images of the viscosity distribution of materials around bipolymer producing cells. An image of the viscosity distribution around a pullulan producing cell of Aureobasidium pullulans is shown as an example. We present results from experiments supporting a linearized model for the motion of a trapped bead in an oscillating harmonic potential. Fluid velocity measurements are tested by comparing to an independent video based measurement. We apply the technique to obtain a 2-D map of the flow past a microscopic wedge and compare to a theoretical solution for the stream lines assuming potential flow. Since the velocity is measured simultaneously with the trap relaxation time, it requires practically no calibration and is independent of the trap stiffness and the particle size.

Paper Details

Date Published: 28 May 2002
PDF: 12 pages
Proc. SPIE 4622, Optical Diagnostics of Living Cells V, (28 May 2002); doi: 10.1117/12.468338
Show Author Affiliations
Boaz A. Nemet, Columbia Univ. (United States)
Nili Harnik, Columbia Univ. (United States)
Mark Cronin-Golomb, Tufts Univ. (United States)

Published in SPIE Proceedings Vol. 4622:
Optical Diagnostics of Living Cells V
Robert C. Leif; Daniel L. Farkas; Robert C. Leif, Editor(s)

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