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Proceedings Paper

Detection of low-molecular-weight analytes by surface plasmon resonance and receptor conformational changes
Author(s): Helen V. Hsieh; Jason E. Gestwicki; J. Bruce Pitner
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Paper Abstract

Low molecular weight molecules are typically very difficult to detect directly in solution using commercially available SPR (surface plasmon resonance) instruments. This is because the mass change on binding is not sufficient to cause a detectable change in refractive index on binding to surface-bound receptors (e.g., antibodies). Some receptors, however, undergo extensive changes in tertiary structure upon binding ligands. Here we present data suggesting conformational changes in surface-bound receptors such as periplasmic binding proteins and calcium-binding proteins can be detected by SPR. This SPR response can be used to monitor specific binding of carbohydrates and calcium even though the molecular weight of these analytes would be difficult to detect using traditional SPR methods. Therefore this approach has potential applications for developing optical biosensors for such small molecules.

Paper Details

Date Published: 23 May 2002
PDF: 8 pages
Proc. SPIE 4624, Optical Diagnostics and Sensing of Biological Fluids and Glucose and Cholesterol Monitoring II, (23 May 2002); doi: 10.1117/12.468310
Show Author Affiliations
Helen V. Hsieh, BD Technologies (United States)
Jason E. Gestwicki, BD Technologies (United States)
J. Bruce Pitner, BD Technologies (United States)


Published in SPIE Proceedings Vol. 4624:
Optical Diagnostics and Sensing of Biological Fluids and Glucose and Cholesterol Monitoring II
Alexander V. Priezzhev; Gerard L. Cote, Editor(s)

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