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Proceedings Paper

Programmable beam-splitter for confocal laser scanning microscopy
Author(s): Holger Birk; Johann Engelhardt; Rafael Storz; Nicole Hartmann; Joachim Bradl; Heinrich Ulrich
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Paper Abstract

In confocal microscopy manifold combinations of dyes are used. The entire wavelength range from near-ultraviolet to near-infrared is used to excite these dyes. Their emission is then detected primarily in the visible range. In addition to the number of dyes, the number of possible excitation laser lines is already large and constantly increasing. Due to the almost unlimited number of possible combinations, programmable devices are required. This is true for excitation-modules, beam-splitters and detection modules. Systems using filters with fixed spectral properties can practically fulfill the requirements for only a very small subset of applications. Programmable devices have been realized for excitation using Acousto Optical Tunable Filters (AOTF's). Freely definable spectral detectors are available for a perfect adaptation to the desired emission bands. The missing link for a completely filter-free design is now introduced using an Acousto Optical Beam Splitter (AOBS).

Paper Details

Date Published: 15 May 2002
PDF: 12 pages
Proc. SPIE 4621, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing IX, (15 May 2002); doi: 10.1117/12.467841
Show Author Affiliations
Holger Birk, Leica Microsystems Heidelberg GmbH (Germany)
Johann Engelhardt, Leica Microsystems Heidelberg GmbH (Germany)
Rafael Storz, Leica Microsystems Heidelberg GmbH (Germany)
Nicole Hartmann, Leica Microsystems Heidelberg GmbH (Germany)
Joachim Bradl, Leica Microsystems Heidelberg GmbH (Germany)
Heinrich Ulrich, Leica Microsystems Heidelberg GmbH (Germany)

Published in SPIE Proceedings Vol. 4621:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing IX
Jose-Angel Conchello; Carol J. Cogswell; Tony Wilson, Editor(s)

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