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Proceedings Paper

High-resolution 3D reconstruction from serial sections: microscope instrumentation, software design, and its implementations
Author(s): Fons J. Verbeek; Paul J. Boon
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Paper Abstract

This paper describes a set-up for high-resolution imaging with a conventional microscope that allows producing a 3D-image set from which a 3D model can be derived. The 3D-image set, is de facto, a gray value voxel model. In order to obtain such 3D image set a good administration needs to be maintained at acquisition and moreover, the acquisition must be realized in two steps. High-resolution images are built of image tiles and sophisticated algorithms are required to build a coherent image from the tiles.

Paper Details

Date Published: 15 May 2002
PDF: 12 pages
Proc. SPIE 4621, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing IX, (15 May 2002); doi: 10.1117/12.467833
Show Author Affiliations
Fons J. Verbeek, Netherlands Institute for Developmental Biology (Netherlands)
Paul J. Boon, Netherlands Institute for Developmental Biology (Netherlands)


Published in SPIE Proceedings Vol. 4621:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing IX
Jose-Angel Conchello; Carol J. Cogswell; Tony Wilson, Editor(s)

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