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Proceedings Paper

Segmentation of confocal microscopic image of insect brain
Author(s): Ming-Jin Wu; Chih-Yang Lin; Yu-Tai Ching
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Paper Abstract

Accurate analysis of insect brain structures in digital confocal microscopic images is valuable and important to biology research needs. The first step is to segment meaningful structures from images. Active contour model, known as snakes, is widely used for segmentation of medical images. A new class of active contour model called gradient vector flow snake has been introduced in 1998 to overcome some critical problems encountered in the traditional snake. In this paper, we use gradient vector flow snake to segment the mushroom body and the central body from the confocal microscopic insect brain images. First, an edge map is created from images by some edge filters. Second, a gradient vector flow field is calculated from the edge map using a computational diffusion process. Finally, a traditional snake deformation process starts until it reaches a stable configuration. User interface is also provided here, allowing users to edit the snake during deformation process, if desired. Using the gradient vector flow snake as the main segmentation method and assist with user interface, we can properly segment the confocal microscopic insect brain image for most of the cases. The identified mushroom and central body can then be used as the preliminary results toward a 3-D reconstruction process for further biology researches.

Paper Details

Date Published: 9 May 2002
PDF: 8 pages
Proc. SPIE 4684, Medical Imaging 2002: Image Processing, (9 May 2002); doi: 10.1117/12.467125
Show Author Affiliations
Ming-Jin Wu, National Chiao Tung Univ. (Taiwan)
Chih-Yang Lin, National Chiao Tung Univ. (Taiwan)
Yu-Tai Ching, National Chiao Tung Univ. (Taiwan)


Published in SPIE Proceedings Vol. 4684:
Medical Imaging 2002: Image Processing
Milan Sonka; J. Michael Fitzpatrick, Editor(s)

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