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Proceedings Paper

Circulating blood cells trigger acute inflammatory responses in the artery wall
Author(s): Alexandra Lucas; Alexander M. Christov; Erbin Dai; Liying Liu; Brodie Nadeau; Jennifer Brown
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Paper Abstract

Whether inflammatory responses are always initiated in the vessel wall, followed by secondary blood cell activation, or are initiated by activated circulating cells that trigger secondary changes in arterial structure remains unproved. In a rabbit angioplasty injury model we studied changes in aortic atherosclerotic plaque after autologous re-infusion of blood cells pre-activated in vitro with thrombin. Fluorescence spectroscopic analysis (FSA) was used to measure membrane fluidity of circulating platelets, as well as quantitative changes in collagen and elastin at the arterial inner surface. The results were correlated with atherosclerotic plaque structural characteristics. Injection of activated circulating blood cells caused a significant increase in fluorescence emission intensity from the abdominal aorta at 450 and 500 nm 3 days later. In blood samples treated with thrombin, membrane fluidity was significantly increased compared to controls. In conclusion, our results indicate that activated circulating blood cells can trigger arterial responses, acting not only as a secondary response to arterial inflammation, but also as a primary activation mechanism.

Paper Details

Date Published: 1 May 2002
PDF: 12 pages
Proc. SPIE 4613, Optical Biopsy IV, (1 May 2002); doi: 10.1117/12.465233
Show Author Affiliations
Alexandra Lucas, John P. Robarts Research Institute/Univ. of Western Ontario and Univ. of Western Ontario (Canada)
Alexander M. Christov, John P. Robarts Research Institute/Univ. of Western Ontario (United States)
Erbin Dai, John P. Robarts Research Institute/Univ. of Western Ontario (Canada)
Liying Liu, John P. Robarts Research Institute/Univ. of Western Ontario (Canada)
Brodie Nadeau, John P. Robarts Research Institute/Univ. of Western Ontario and Univ. of Western Ontario (Canada)
Jennifer Brown, Univ. of Western Ontario (Canada)


Published in SPIE Proceedings Vol. 4613:
Optical Biopsy IV
Robert R. Alfano, Editor(s)

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