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Proceedings Paper

Alternative method for simulating IPSF in two-photon laser scanning microscopy
Author(s): YongJun Zhou; DeQiang Chen; Wenhao Huang; Andong Xia
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Paper Abstract

The refractive-index mismatch induced by coverslips will lead to the distortion of two-photon laser scanning microscopy (TPLSM) images. Such distortion will result in the elongation of the intensity point spread function (IPSF). In order to predict the trend and extent of the distortion, we develop a new data proceeding method which combines Torok’s theory with the experimental value. This method considers the fluorescence quantum efficiency of the sample together with the detection efficiency of the system. By calculating IPSF and using our method to proceed the data, the simulation results are quite consistent with experimental results.

Paper Details

Date Published: 20 September 2002
PDF: 8 pages
Proc. SPIE 4927, Optical Design and Testing, (20 September 2002); doi: 10.1117/12.464324
Show Author Affiliations
YongJun Zhou, Univ. of Science and Technology of China (China)
DeQiang Chen, Univ. of Science and Technology of China (China)
Wenhao Huang, Univ. of Science and Technology of China (China)
Andong Xia, Univ. of Science and Technology of China and Institute of Chemistry (China)

Published in SPIE Proceedings Vol. 4927:
Optical Design and Testing
Zhicheng Weng; Jose M. Sasian; Yongtian Wang, Editor(s)

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