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Proceedings Paper

Fluorescence in the fiber optic HPTLC-method
Author(s): Karl-Friedrich Klein; Bernd Spangenberg; Bjoern Arenz
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Paper Abstract

The sample analysis with Thin Layer Chromatography is an off-line method, in contrast to other separation methods. In addition to the sample preparation, the key-step is the in- situ densitometry for qualitative or quantitative purposes by measuring the optical density of the separated spots directly on the plate. With a newly developed HPTLC-system using fiber-optic and diode-array detection, simultaneous measurements at different wavelengths can be performed. The fiber-optic assembly consists of UV-improved silica-based fibers transporting the light and illuminating the surface of the TLC-plate. The light is scattered and reflected from the surface and finally collected by the reading fibers, carrying the relevant information. Up to now, only the absorption mode was used. However, using fluorescent substances, the light-power in a well-defined wavelength- region can be increased: the collected light from the illuminated spot of a substance on the TLC-plate is higher than the reference probe without substances. Fluorescent measurements are possible without filters or special lamps and the improvement of signal-to-noise ratios is significant. Due to the detailed structure of the PAH's absorption- and fluorescence spectra, diode-array HPTLC makes quantification of 16 PAH, recommended for monitoring by EPA, on one track possible. There is an additional advantage: although the spatial resolution of the TLC-separation of PAH is not sufficient for in situ quantitative densitometric analysis each of the 16 compounds can be quantified with the new method using suitable wavelengths.

Paper Details

Date Published: 26 March 2002
PDF: 8 pages
Proc. SPIE 4616, Optical Fibers and Sensors for Medical Applications II, (26 March 2002); doi: 10.1117/12.463816
Show Author Affiliations
Karl-Friedrich Klein, Fachhochschule Giessen-Friedberg (Germany)
Bernd Spangenberg, Fachhochschule Offenburg (Germany)
Bjoern Arenz, Friedrich-Schiller-Univ. Jena (Germany)


Published in SPIE Proceedings Vol. 4616:
Optical Fibers and Sensors for Medical Applications II
Israel Gannot, Editor(s)

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